Catalog number: 65C-CE0305
Full name: Streptavidin protein Cy3.5
Size: 1 mg
Antibody Subtype: Conjugated Proteins
Area of research: Miscellaneous
Reactivity: Streptomyces avidinii
Type of protein: Binding Protein
Method of Purification: Streptavidin protein (Cy3.5) was purified by Protein A affinity chromatography.
Form & Buffer: Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
Storage: Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping conditions: Blue Ice
Tested for: FC; IF
Usage Recommendations: DC: 1:200-1:400, IF: 1:100-1:1,000
Properties: Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Streptavidin protein .5 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This fitzgerald antibody is well suited for fluorescent microscopy.
Conjugation: cy3 conjugation kit