About Streptavidin protein FITC

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Catalog number: 65C-CE0308

Full name: Streptavidin protein FITC

Size: 1 mg

Supplier:  fitzgerald

Price: 315.00

Category : Proteins

Antibody Subtype : Conjugated Proteins

Area of research : Miscellaneous

Reactivity : Streptomyces avidinii

Conjugate/Tag : FITC

Type of protein : Binding Protein

Method of Purification : Streptavidin protein (FITC) was purified by Protein A affinity chromatography.

Form & Buffer : Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.

Storage : Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping conditions : Blue Ice

Tested for : ELISA; FC; IF

Usage Recommendations : ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000

Properties : This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.

Conjugation : Anti-FITC Antibody