Catalog number: 65C-CE0308
Full name: Streptavidin protein FITC
Size: 1 mg
Antibody Subtype: Conjugated Proteins
Area of research: Miscellaneous
Reactivity: Streptomyces avidinii
Type of protein: Binding Protein
Method of Purification: Streptavidin protein (FITC) was purified by Protein A affinity chromatography.
Form & Buffer: Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
Storage: Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping conditions: Blue Ice
Tested for: ELISA; FC; IF
Usage Recommendations: ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000
Properties: This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
Conjugation: Anti-FITC Antibody